Virginia Commonwealth University

Make it real.

Featured Researcher

Research Expertise and Focus

  • Immunoglobulin E (IgE)-mediated allergic disease and immunologic cell receptors
  • Investigating how the immunity that causes asthma may be regulated by molecules from the nervous system. One of seven senior investigator awards for asthma research. Funded 2011-2013 by the American Asthma Foundation, at $250,000 a year. The application involves novel studies probing the role of the metalloprotease ADAM10 in asthma.
  • Regulation of the IgE response. Work performed in the senior author’s laboratory was supported by National Institutes of Health grants R01AI18697 and U19AI077435, project 2. Senior author, principal investigator

Professional Highlights

Membership and Leadership

  • AADCRC Study Section, 2011
  • Allergy, Immunology and Transplantation (AITRC) study section, 2000-2002

Awards

  • University Award of Excellence, VCU, 2012
  • Distinguished Mentor Award, VCU School of Medicine, 2011 and 2001

Editorial

  • Section editor, Cutting Edge, 2004-2007; Journal of Immunology, 1991-1993
  • Ad hoc reviewer, J. Immunol., Mol. Immunol., Proc. Natl. Acad. Sci., J. Biol. Chem., J. Clin. Invest., others

Education and Training

  • Ph. D. in immunology and B.S., West Virginia University, Morgantown, W.Va.
  • Research fellowships in immunology: University of Manitoba, Winnipeg, Canada; VCU Medical Center
  • Senior Research Fellow of the American Asthma Foundation

Selected Publications

Oyeniran C, Sturgill JL, Hait NC, Huang WC, Avni D, Maceyka M, Newton J, Allegood JC, Montpetit A, Conrad DH, Milstien S, Spiegel S. Aberrant ORM  (yeast)-like protein isoform 3 (ORMDL3) expression dysregulates ceramide homeostasis in cells and ceramide exacerbates allergic asthma in mice. J Allergy Clin Immunol. 2015 Oct;136(4):1035-1046.e6. doi: 10.1016/j.jaci.2015.02.031. Epub 2015 Apr 2. PubMed PMID: 25842287; PubMed Central PMCID: PMC4591101.

Damle SR, Martin RK, Cross JV, Conrad DH. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol. 2016 Apr 6. doi: 10.1038/mi.2016.29. [Epub ahead of print]

Cooley LF, Martin RK, Zellner HB, Irani AM, Uram-Tuculescu C, El Shikh ME, Conrad DH. Increased B cell ADAM10 in allergic patients and Th2 prone mice. PLoS One. 2015 May 1;10(5):e0124331. PMCID: PMC4416757

Martin RK, Brooks KB, Henningsson F, Heyman B, Conrad DH. Antigen transfer from exosomes to dendritic cells as an explanation for the immune enhancement seen by IgE immune complexes. PLoS One. 2014 Oct 20;9(10):e110609. PMCID: PMC4203810

Faber TW, Pullen NA, Fernando JF, Kolawole EM, McLeod JJ, Taruselli M, Williams KL, Rivera KO, Barnstein BO, Conrad DH, Ryan JJ. ADAM10 is required for SCF-induced mast cell migration. Cell Immunol. 2014 Jul;290(1):80-88. PMCID: PMC4128322

Martin RK, Saleem SJ, Folgosa L, Zellner HB, Damle SR, Nguyen GK, Ryan JJ, Bear HD, Irani AM, Conrad DH. Mast cell histamine promotes the immunoregulatory activity of myeloid-derived suppressor cells. J Leukoc Biol. 2014 Jul;96(1):151-159. PMCID: PMC4056279

Morales JK, Saleem SJ, Martin RK, Saunders BL, Barnstein BO, Faber TW, Pullen NA, Kolawole EM, Brooks KB, Norton SK, Sturgill J, Graham L, Bear HD, Urban JF,Jr, Lantz CS, Conrad DH, Ryan JJ. Myeloid-derived suppressor cells enhance IgE-mediated mast cell responses. J Leukoc Biol. 2014 Apr;95(4):643-650. PMCID: PMC3958743

Chaimowitz NS, Falanga YT, Ryan JJ, Conrad DH. Fyn kinase is required for optimal humoral responses. PLoS One. 2013 Apr 8;8(4):e60640. PMCID: PMC3620480

Folgosa L, Zellner HB, El Shikh ME, Conrad DH. Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-alpha shedding. J Immunol. 2013 Dec 15;191(12):5951-5958. PMCID: PMC3863601

Fernando J, Faber TW, Pullen NA, Falanga YT, Kolawole EM, Oskeritzian CA, Barnstein BO, Bandara G, Li G, Schwartz LB, Spiegel S, Straus DB, Conrad DH, Bunting KD, Ryan JJ. Genotype-dependent effects of TGF-beta1 on mast cell function: Targeting the Stat5 pathway. J Immunol. 2013 Nov 1;191(9):4505-4513. PMCID: PMC3846427

Price MM, Oskeritzian CA, Falanga YT, Harikumar KB, Allegood JC, Alvarez SE, Conrad D, Ryan JJ, Milstien S, Spiegel S. A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell-dependent murine model of allergic asthma. J Allergy Clin Immunol. 2013 Feb;131(2):501-11.e1. PMCID: PMC3563730

Norton SK, Wijesinghe DS, Dellinger A, Sturgill J, Zhou Z, Barbour S, Chalfant C, Conrad DH, Kepley CL. Epoxyeicosatrienoic acids are involved in the C(70) fullerene derivative-induced control of allergic asthma. J Allergy Clin Immunol. 2012 Sep;130(3):761-769.e2. PMCID: PMC3955256

Saleem SJ, Martin RK, Morales JK, Sturgill JL, Gibb DR, Graham L, Bear HD, Manjili MH, Ryan JJ, Conrad DH. Cutting edge: Mast cells critically augment myeloid-derived suppressor cell activity. J Immunol. 2012 Jul 15;189(2):511-515. PMCID: PMC3392490

Falanga YT, Chaimowitz NS, Charles N, Finkelman FD, Pullen NA, Barbour S, Dholaria K, Faber T, Kolawole M, Huang B, Odom S, Rivera J, Carlyon J, Conrad DH, Spiegel S, et al. Lyn but not fyn kinase controls IgG-mediated systemic anaphylaxis. J Immunol. 2012 May 1;188(9):4360-4368. PMCID: PMC3536057

Gomez-Arroyo J, Saleem SJ, Mizuno S, Syed AA, Bogaard HJ, Abbate A, Taraseviciene-Stewart L, Sung Y, Kraskauskas D, Farkas D, Conrad DH, Nicolls MR, Voelkel NF. A brief overview of mouse models of pulmonary arterial hypertension: Problems and prospects. Am J Physiol Lung Cell Mol Physiol. 2012 May 15;302(10):L977-91. PMCID: PMC3774477

Zuo D, Yu X, Guo C, Yi H, Chen X, Conrad DH, Guo TL, Chen Z, Fisher PB, Subjeck JR, Wang XY. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. Faseb j. 2012 Apr;26(4):1493-1505. PMCID: PMC3316907

Miller DP, Bell JK, McDowell JV, Conrad DH, Burgner JW, Heroux A, Marconi RT. Structure of factor H-binding protein B (FhbB) of the periopathogen, treponema denticola: Insights into progression of periodontal disease. J Biol Chem. 2012 Apr 13;287(16):12715-12722. PMCID: PMC3339992

Chaimowitz NS, KangDJ, Dean LM, Conrad DH. ADAM10 regulates transcription factor expression required for plasma cell function. PLoS One. 2012; 7(8): e42694. PMCID: PMC3411801

Suzuki M, El-Hage N, Zou S, Hahn YK, Sorrell ME, Sturgill JL, Conrad DH, Knapp PE, Hauser KF. Fractalkine/CX3CL1 protects striatal neurons from synergistic morphine and HIV-1 tat-induced dendritic losses and death. Mol Neurodegener. 2011 Nov 17;678-1326-6-78. PMCID: PMC3287119

Chaimowitz NS, Martin RK, Cichy J, Gibb DR, Patil P, Kang DJ, Farnsworth J, Butcher EC, McCright B, Conrad DH. A disintegrin and metalloproteinase 10 regulates antibody production and maintenance of lymphoid architecture. J.Immunol. 2011 Nov 15;187(10):5114-5122. PMCID: PMC4006936

Manque PA, Tenjo F, Woehlbier U, Lara AM, Serrano MG, Xu P, Alves JM, Smeltz RB, Conrad DH, Buck GA. Identification and immunological characterization of three potential vaccinogens against Cryptosporidium species. Clin Vaccine Immunol. 2011 Nov;18(11):1796-802. PMCID: PMC3209039

Wang J, Cheng X, Xiang MX, Alanne-Kinnunen M, Wang JA, Chen H, He A, Sun X, Lin Y, Tang TT, Tu X, Sjoberg S, Sukhova GK, Liao YH, Conrad DH, et al. IgE stimulates human and mouse arterial cell apoptosis and cytokine expression and promotes atherogenesis in apoe-/- mice. J Clin Invest. 2011 Sep;121(9):3564-3577. PMCID: PMC3163955

Mathews JA, Ford J, Norton S, Kang D, Dellinger A, Gibb DR, Ford AQ, Massay H, Kepley CL, Scherle P, Keegan AD, Conrad DH. A potential new target for asthma therapy: A disintegrin and metalloprotease 10 (ADAM10) involvement in murine experimental asthma. Allergy. 2011 Sep;66(9):1193-1200. PMCID: PMC3963393

Schlosburg JE, O’Neal ST, Conrad DH, Lichtman AH. CB1 receptors mediate rimonabant-induced pruritic responses in mice: Investigation of locus of action. Psychopharmacology (Berl). 2011 Aug;216(3):323-331. PMCID: PMC3606913

Saleem SJ, Conrad DH. Hematopoietic cytokine-induced transcriptional regulation and notch signaling as modulators of MDSC expansion. Int Immunopharmacol. 2011 Jul;11(7):808-815. PMCID: PMC3119497

Gibb DR, Saleem SJ, Chaimowitz NS, Mathews J, Conrad DH. The emergence of ADAM10 as a regulator of lymphocyte development and autoimmunity. Mol Immunol. 2011 Jun;48(11):1319-27.  PMCID: PMC3083477

Sturgill JL, Mathews J, Scherle P, Conrad DH. Glutamate signaling through the kainate receptor enhances human immunoglobulin production. J Neuroimmunol. 2011 Apr;233(1-2):80-89. PMCID: PMC3074000

Mathews JA, Gibb DR, Chen BH, Scherle P, Conrad DH. CD23 sheddase A disintegrin and metalloproteinase 10 (ADAM10) is also required for CD23 sorting into B cell-derived exosomes. J Biol Chem. 2010 Nov 26;285(48):37531-37541. PMCID: PMC2988358

Ge X, Kitten T, Munro CL, Conrad DH, Xu P. Pooled protein immunization for identification of cell surface antigens in streptococcus sanguinis. PLoS One. 2010 Jul 26;5(7):e11666. PMCID: PMC2909906

Gibb DR, El Shikh M, Kang DJ, Rowe WJ, El Sayed R, Cichy J, Yagita H,Tew JG, Dempsey PJ, Crawford HC, Conrad DH. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo.J Exp Med. 2010 Mar 15;207(3):623-35. PMCID: PMC2839139

Conrad DH, Gibb DR, Sturgill J. Regulation of the IgE response. F1000 Biol Rep. 2010 Feb 24;210.3410/B2-14. PMCID: PMC2948365

Ford JW, Sturgill JL, Conrad DH. 129/SvJ mice have mutated CD23 and hyper IgE. Cell Immunol. 2009 254(2):124-134. PMCID: PMC2643045

Conrad DH, Goyette J, Thomas PS. Proteomics as a method for early detection of cancer: A review of proteomics, exhaled breath condensate, and lung cancer screening. J Gen Intern Med. 2008 Jan;23 Suppl 178-84. PMCID: PMC2150625

Caven TH, Sturgill JL, Conrad DH. BCR ligation antagonizes the IL-21 enhancement of anti-CD40/IL-4 plasma cell differentiation and IgE production found in low density human B cell cultures. Cell Immunol. 2007 May;247(1):49-58. PMCID: PMC2075084

Chen BH, Ma C, Caven TH, Chan-Li Y, Beavil A, Beavil R, Gould H, Conrad DH. Necessity of the stalk region for immunoglobulin E interaction with CD23. Immunology. 2002 Nov;107(3):373-381. PMCID: PMC1782806

Rabah D, Conrad DH. Effect of cell density on in vitro mouse immunoglobulin E production. Immunology. 2002 Aug;106(4):503-510. PMCID: PMC1782743

Kilmon MA, Mayer RJ, Marshall LA, Conrad DH. Metalloprotease inhibitor-mediated inhibition of mouse immunoglobulin production. Immunology. 2001 Mar;102(3):281-288. PMCID: PMC1783188

Yang PC, Berin MC, Yu LC, Conrad DH, Perdue MH. Enhanced intestinal transepithelial antigen transport in allergic rats is mediated by IgE and CD23 (FcepsilonRII). J Clin Invest. 2000 Oct;106(7):879-886.PMCID: PMC517813

Conrad DH, Ben-Sasson SZ, Le Gros G, Finkelman FD, Paul WE. Infection with nippostrongylus brasiliensis or injection of anti-IgD antibodies markedly enhances fc-receptor-mediated interleukin 4 production by non-B, non-T cells. J Exp Med. 1990 May 1;171(5):1497-1508. PMCID: PMC2187898

Ben-Sasson SZ, Le Gros G, Conrad DH, Finkelman FD, Paul WE. Cross-linking fc receptors stimulate splenic non-B, non-T cells to secrete interleukin 4 and other lymphokines. Proc Natl Acad Sci U.S.A. 1990 Feb;87(4):1421-1425. PMCID: PMC53487

Bettler B, Hofstetter H, Rao M, Yokoyama WM, Kilchherr F, Conrad DH. Molecular structure and expression of the murine lymphocyte low-affinity receptor for IgE (fc epsilon RII). Proc Natl Acad Sci U.S.A. 1989 Oct;86(19):7566-7570. PMCID: PMC298106

Hudak SA, Gollnick SO, Conrad DH, Kehry MR. Murine B-cell stimulatory factor 1 (interleukin 4) increases expression of the fc receptor for IgE on mouse B cells. Proc Natl Acad Sci U.S.A. 1987 Jul;84(13):4606-4610. PMCID: PMC305139

Lee WT, Conrad DH. The murine lymphocyte receptor for IgE. II. characterization of the multivalent nature of the B lymphocyte receptor for IgE  J Exp Med. 1984 Jun 1;159(6):1790-1795. PMCID: PMC2187318

Carlson P, Ruddy S, Conrad DH. Solubilization of an activity regulating C3b function from raji cell membranes. Immunology. 1982 May;46(1):163-173. PMCID: PMC1555332

Froese A, Helm RM, Conrad DH, Isersky C, Ishizaka T. Comparison of the receptors for IgE of various rat basophilic leukaemia cell lines. II. studies with different anti-receptor antisera. Immunology. 1982 May;46(1):117-123. PMCID: PMC1555351

Froese A, Helm RM, Conrad DH, Isersky C, Ishizaka T, Kulczycki A,Jr. Comparison of the receptors for IgE of various rat basophilic leukaemia cell lines. I. receptors isolated by IgE-sepharose and IgE and anti-IgE. Immunology. 1982 May;46(1):107-116. PMCID: PMC1555343

Carlo JR, Spitznagel JK, Studer EJ, Conrad DH, Ruddy S. Cleavage of membrane bound C3bi, an intermediate of the third component of complement, to C3c and C3d-like fragments by crude leucocyte lysosomal lysates and purified leucocyte elastase. Immunology. 1981 Oct;44(2):381-391. PMCID: PMC1555206

Conrad DH, Carlo JR, Ruddy S. Interaction of beta1H globulin with cell-bound C3b: Quantitative analysis of binding and influence of alternative pathway components on binding. J Exp Med. 1978 Jun 1;147(6):1792-1805. PMCID: PMC2184316